niels geijsen (Addgene inc)
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niels geijsen
Niels Geijsen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/niels+geijsen/CRE+recombinase+(Plasmid+%2362730)/pmc12992809-357-13-15
Average 96 stars, based on 354 article reviews
Niels Geijsen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/niels+geijsen/CRE+recombinase+(Plasmid+%2362730)/pmc12992809-357-13-15
Average 96 stars, based on 354 article reviews
niels geijsen - by Bioz Stars,
2026-10
96/100 stars
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Clone Assay:Article Title: Quiescent cell re-entry is limited by macroautophagy-induced lysosomal damage. Article Snippet: .. The sequences listed in the ‘‘key resources table’’ were cloned in pLENTIGUIDE F-E, which was modified from lentiguide-PURO with the F-E modification for increased potency used for CRISPRi.101,102 pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro was a gift from Marco Jost & Jonathan Weissman (Addgene plasmid # 188768; http:// n2t.net/addgene:188768; RRID:Addgene_188768), mAG-GAL3 was a gift from Modification:Article Title: Quiescent cell re-entry is limited by macroautophagy-induced lysosomal damage. Article Snippet: .. The sequences listed in the ‘‘key resources table’’ were cloned in pLENTIGUIDE F-E, which was modified from lentiguide-PURO with the F-E modification for increased potency used for CRISPRi.101,102 pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro was a gift from Marco Jost & Jonathan Weissman (Addgene plasmid # 188768; http:// n2t.net/addgene:188768; RRID:Addgene_188768), mAG-GAL3 was a gift from Plasmid Preparation:Article Title: Quiescent cell re-entry is limited by macroautophagy-induced lysosomal damage. Article Snippet: .. The sequences listed in the ‘‘key resources table’’ were cloned in pLENTIGUIDE F-E, which was modified from lentiguide-PURO with the F-E modification for increased potency used for CRISPRi.101,102 pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro was a gift from Marco Jost & Jonathan Weissman (Addgene plasmid # 188768; http:// n2t.net/addgene:188768; RRID:Addgene_188768), mAG-GAL3 was a gift from Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes Article Snippet: .. To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63, and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817 ; PRID:Addgene_1817). mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes. Article Snippet: Next, the amplified segment was inserted into an entry vector pENTR1A (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17398; http://n2t.net/addgene:17398; PRID:Addgene_17398)67 by golden gate assembly method.68 The plasmid obtained was then recombined with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17452; http://n2t.net/addgene:17452; PRID:Addgene_17452)67 using a gateway LR clonase enzyme (ThermoFisher Scientific, 11791100) to achieve the expression vector pLenti-CMV-N-Flag-APEX2-emGFP-CD63-Puro- DEST. .. For GFP display at the surface of EVs, emGFP was inserted at the second extracellular loop of CD63 following a reported strategy, to generate pLenti-CMV-N-CD63-emGFP-CD63-CPuro-DEST.69 To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63 and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817; PRID:Addgene_1817).70 mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Article Title: A combinatorial synthetic strategy for developing genome-editing protein-delivery agents targeting mouse retina Article Snippet: .. Plasmid encoding Cre recombinase for expression in Escherichia coli was a gift from Article Title: dbGuide: a database of functionally validated guide RNAs for genome editing in human and mouse cells. Article Snippet: .. Cas9 protein was produced using plasmid Addgene62731, a gift from Expressing:Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes Article Snippet: .. To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63, and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817 ; PRID:Addgene_1817). mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes. Article Snippet: Next, the amplified segment was inserted into an entry vector pENTR1A (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17398; http://n2t.net/addgene:17398; PRID:Addgene_17398)67 by golden gate assembly method.68 The plasmid obtained was then recombined with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17452; http://n2t.net/addgene:17452; PRID:Addgene_17452)67 using a gateway LR clonase enzyme (ThermoFisher Scientific, 11791100) to achieve the expression vector pLenti-CMV-N-Flag-APEX2-emGFP-CD63-Puro- DEST. .. For GFP display at the surface of EVs, emGFP was inserted at the second extracellular loop of CD63 following a reported strategy, to generate pLenti-CMV-N-CD63-emGFP-CD63-CPuro-DEST.69 To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63 and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817; PRID:Addgene_1817).70 mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Article Title: A combinatorial synthetic strategy for developing genome-editing protein-delivery agents targeting mouse retina Article Snippet: .. Plasmid encoding Cre recombinase for expression in Escherichia coli was a gift from Cloning:Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes Article Snippet: .. To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63, and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817 ; PRID:Addgene_1817). mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes. Article Snippet: Next, the amplified segment was inserted into an entry vector pENTR1A (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17398; http://n2t.net/addgene:17398; PRID:Addgene_17398)67 by golden gate assembly method.68 The plasmid obtained was then recombined with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17452; http://n2t.net/addgene:17452; PRID:Addgene_17452)67 using a gateway LR clonase enzyme (ThermoFisher Scientific, 11791100) to achieve the expression vector pLenti-CMV-N-Flag-APEX2-emGFP-CD63-Puro- DEST. .. For GFP display at the surface of EVs, emGFP was inserted at the second extracellular loop of CD63 following a reported strategy, to generate pLenti-CMV-N-CD63-emGFP-CD63-CPuro-DEST.69 To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63 and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817; PRID:Addgene_1817).70 mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Amplification:Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes Article Snippet: .. To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63, and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817 ; PRID:Addgene_1817). mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes. Article Snippet: Next, the amplified segment was inserted into an entry vector pENTR1A (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17398; http://n2t.net/addgene:17398; PRID:Addgene_17398)67 by golden gate assembly method.68 The plasmid obtained was then recombined with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17452; http://n2t.net/addgene:17452; PRID:Addgene_17452)67 using a gateway LR clonase enzyme (ThermoFisher Scientific, 11791100) to achieve the expression vector pLenti-CMV-N-Flag-APEX2-emGFP-CD63-Puro- DEST. .. For GFP display at the surface of EVs, emGFP was inserted at the second extracellular loop of CD63 following a reported strategy, to generate pLenti-CMV-N-CD63-emGFP-CD63-CPuro-DEST.69 To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63 and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817; PRID:Addgene_1817).70 mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Sequencing:Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes Article Snippet: .. To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63, and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817 ; PRID:Addgene_1817). mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes. Article Snippet: Next, the amplified segment was inserted into an entry vector pENTR1A (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17398; http://n2t.net/addgene:17398; PRID:Addgene_17398)67 by golden gate assembly method.68 The plasmid obtained was then recombined with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17452; http://n2t.net/addgene:17452; PRID:Addgene_17452)67 using a gateway LR clonase enzyme (ThermoFisher Scientific, 11791100) to achieve the expression vector pLenti-CMV-N-Flag-APEX2-emGFP-CD63-Puro- DEST. .. For GFP display at the surface of EVs, emGFP was inserted at the second extracellular loop of CD63 following a reported strategy, to generate pLenti-CMV-N-CD63-emGFP-CD63-CPuro-DEST.69 To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63 and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817; PRID:Addgene_1817).70 mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Polymerase Chain Reaction:Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes. Article Snippet: Next, the amplified segment was inserted into an entry vector pENTR1A (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17398; http://n2t.net/addgene:17398; PRID:Addgene_17398)67 by golden gate assembly method.68 The plasmid obtained was then recombined with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17452; http://n2t.net/addgene:17452; PRID:Addgene_17452)67 using a gateway LR clonase enzyme (ThermoFisher Scientific, 11791100) to achieve the expression vector pLenti-CMV-N-Flag-APEX2-emGFP-CD63-Puro- DEST. .. For GFP display at the surface of EVs, emGFP was inserted at the second extracellular loop of CD63 following a reported strategy, to generate pLenti-CMV-N-CD63-emGFP-CD63-CPuro-DEST.69 To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63 and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817; PRID:Addgene_1817).70 mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Generated:Article Title: Endocytosis of Extracellular Vesicles and Release of Their Cargo from Endosomes. Article Snippet: Next, the amplified segment was inserted into an entry vector pENTR1A (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17398; http://n2t.net/addgene:17398; PRID:Addgene_17398)67 by golden gate assembly method.68 The plasmid obtained was then recombined with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman; Addgene, plasmid# 17452; http://n2t.net/addgene:17452; PRID:Addgene_17452)67 using a gateway LR clonase enzyme (ThermoFisher Scientific, 11791100) to achieve the expression vector pLenti-CMV-N-Flag-APEX2-emGFP-CD63-Puro- DEST. .. For GFP display at the surface of EVs, emGFP was inserted at the second extracellular loop of CD63 following a reported strategy, to generate pLenti-CMV-N-CD63-emGFP-CD63-CPuro-DEST.69 To generate the expression vector pLenti-CMV-CD63-mRFP using the same cloning strategy, CD63 was amplified from N-Flag-Apex2-emGFP-CD63 and mRFP sequence was amplified from Lamp1-mRFP plasmid (a gift from Walther Mothes; Addgene, plasmid# 1817; http://n2t.net/addgene:1817; PRID:Addgene_1817).70 mAzami-Green (mAG)-galectin 3 (Gal3) plasmid was a gift from Produced:Article Title: dbGuide: a database of functionally validated guide RNAs for genome editing in human and mouse cells. Article Snippet: .. Cas9 protein was produced using plasmid Addgene62731, a gift from |